| Compound | Results | Value from t-test significance table (α = 0.05) |
| Cholesterol | ||
| Linear range (μg·mL−1) | 5.0 - 80.0 | |
| n | 25 | |
| Slope (b) | 0.106 | |
| Intercept (a) | −0.016 | |
| Correlation coefficient (r) | 0.9997 | |
| CV% | 2.3 | |
| Confidence limits of a | ±0.234 | |
| t-student | −0.14 | 2.07 |
| ANOVA test: | ||
| F-test (Regression) | 34426 | 4.35 |
| F-test (Deviation from linearity) | 1.677 | 3.10 |
| 7-DHC | ||
| Linear range (μg·mL−1) | 0.2 - 5.0 | |
| n | 25 | |
| Slope (b) | 0.081 | |
| Intercept (a) | 0.021 | |
| Correlation coefficient (r) | 0.9988 | |
| CV% | 5.1 | |
| Confidence limits of a | ±0.020 | |
| t-student | 2.04 | 2.07 |
| ANOVA test: | ||
| F-test (Regression) | 9469 | 4.35 |
| F-test (Deviation from linearity) | 5.575 | 3.10 |